| Biography | |
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 Dr. Bhupendra Koul Lovely Professional University, Pakistan |
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| Biography:
A teacher and researcher, presently working as an Assistant Professor in Department of Biotechnology, Lovely Professional University, Punjab. During my Ph.D., I have worked on introduction and expression of modified full-length and truncated versions of Bt-cry1Ab and cry1Ac genes in tomato for developing stable transgenic lines resistant to lepidopteran insect and evaluating the comparative performance of full-length and truncated versions of cry1Ac gene for stability and efficacy of insecticidal toxin in transgenic plants. In order to address this issue, the basic technique of plant tissue culture, gene-cloning, Agrobacterium-mediated transformation, molecular characterization of transgene expression, population study of the transgenics, including immunological and insect bioassays, were performed. An efficient protocol of Agrobacterium-mediated genetic transformation in tomato for rapid and high frequency recovery of non-chimeric transgenic plants has been optimized. I have screened and selected few highly promising transgenic events of tomato plant expressing modified Bt-cry1Ab and cry1Ac genes showing complete protection against H. armigera and S. litura. Several stable transgenic tomato plants resistant to plant pathogens, through expression of protein transcription co-activator taf4b gene (patent published) and chitinolytic protein toxic to whiteflies, using the optimized protocol, have also developed. I have also developed the art of research methodology, analyzing scientific data and writing manuscripts and have seventeen publications to my credit, and few are under review.
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