Nicole Jaffrezic-Renault_CIC 2020

Biography
Prof. Nicole Jaffrezic Renault University of Lyon, France
Title: Seaweeds and shellfish derived polysaccharides for the design of biomimetic sensor
Abstract: Over the past two decades numerous studies have been reported on seaweeds and shellfish derived polysaccharides for biomedical and biological applications (tissue engineering, drug delivery, wound healing, and biosensor) [1]. A voltammetric biosensor was obtained for the first time by encapsulation of glucose oxidase in the chitosan-kappa-carrageenan polyelectrolyte complex. This glucose biosensor presents a large linear range - from 10 µM to10 mM -, a detection limit of 10 µM, a shelf lifetime of more than one month. There was no effect of interfering substances such as ascorbic acid and uric acid on the anodic signal of hydrogen peroxide at 0.6 V, due to charge effect of the biosensor surface. Using this biosensor, trace amounts of glucose were detected in spiked saliva samples [2]. The analytical features a laccase/chitosan-lambda-carrageenan based voltammetric biosensor for catechol detection are the following ones. The linear range was from 10-9 M to 10-15 M with a sensitivity of 0.22 µA/µM and a limit of detection of 10-16 M. The laccase biosensor exhibits good repeatability (RSD 5.4%) and stability (four weeks). The applicability of the developed biosensor was tested to evaluate the total polyphenolic content in natural oil samples. Voltammetric sensor based on electrodeposited molecularly imprinted chitosan film on BDD electrodes was designed for catechol detection. Polymeric chitosan matrix has been electrodeposited by chronoamperometry in the presence of catechol followed by elution with 0.1 M KCl on a boron doped diamond (BDD) (1). The electrochemical response of the sensor analyzed by cyclic voltammetry (CV) indicates that the sensor shows an excellent reproducibility and repeatability to catechol detection in the range of 0 to 25 µM, with a detection limit of 5.7•10-7 M and high selectivity to catechol recognition versus different phenolic compounds. The results obtained in a red wine denotes the extraordinary capability to detect catechol in a complex matrix. These three examples show the high potentiality of seaweeds and shellfish derived polysaccharides in sensor design. Associated with 3D printing of biosourced polymers, the biodegradability of these biosensors could be ensured.

Biography

Prof. Nicole Jaffrezic Renault
University of Lyon, France

Title: Seaweeds and shellfish derived polysaccharides for the design of biomimetic sensor

Abstract:

Over the past two decades numerous studies have been reported on seaweeds and shellfish derived polysaccharides for biomedical and biological applications (tissue engineering, drug delivery, wound healing, and biosensor) [1].

A voltammetric biosensor was obtained for the first time by encapsulation of glucose oxidase in the chitosan-kappa-carrageenan polyelectrolyte complex. This glucose biosensor presents a large linear range - from 10 µM to10 mM -, a detection limit of 10 µM, a shelf lifetime of more than one month. There was no effect of interfering substances such as ascorbic acid and uric acid on the anodic signal of hydrogen peroxide at 0.6 V, due to charge effect of the biosensor surface. Using this biosensor, trace amounts of glucose were detected in spiked saliva samples [2].

The analytical features a laccase/chitosan-lambda-carrageenan based voltammetric biosensor for catechol detection are the following ones. The linear range was from 10-9 M to 10-15 M with a sensitivity of 0.22 µA/µM and a limit of detection of 10-16 M. The laccase biosensor exhibits good repeatability (RSD 5.4%) and stability (four weeks). The applicability of the developed biosensor was tested to evaluate the total polyphenolic content in natural oil samples.

Voltammetric sensor based on electrodeposited molecularly imprinted chitosan film on BDD electrodes was designed for catechol detection. Polymeric chitosan matrix has been electrodeposited by chronoamperometry in the presence of catechol followed by elution with 0.1 M KCl on a boron doped diamond (BDD) (1). The electrochemical response of the sensor analyzed by cyclic voltammetry (CV) indicates that the sensor shows an excellent reproducibility and repeatability to catechol detection in the range of 0 to 25 µM, with a detection limit of 5.7•10-7 M and high selectivity to catechol recognition versus different phenolic compounds. The results obtained in a red wine denotes the extraordinary capability to detect catechol in a complex matrix.

These three examples show the high potentiality of seaweeds and shellfish derived polysaccharides in sensor design. Associated with 3D printing of biosourced polymers, the biodegradability of these biosensors could be ensured.